rabbit pab homer 1/2/3 antibody (Synaptic Systems)
Structured Review

Rabbit Pab Homer 1/2/3 Antibody, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+pab+homer+2+antibody/rabbit+pab+antibody/pmc08173732-17-3-8
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains"
Article Title: Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains
Journal: Molecular Brain
doi: 10.1186/s13041-021-00799-2
Figure Legend Snippet: Specifics on primary antibodies
Techniques Used: Membrane
Figure Legend Snippet: Comparison on structural preservation upon different fixation conditions and subsequent treatments. Images show synaptic profiles from 3 week-old dissociated hippocampal cultures without ( a , b ) or with ( c , d ) immunogold labeling. Clusters of synaptic vesicles (SV in a ) and the postsynaptic density (PSD, edges of which marked with arrows in a ) are characteristics of glutamatergic asymmetric synapses. The fixation treatments were 4% PF in PBS for 45 min ( a ), 4% glutaraldehyde in 0.1 M cacodylate buffer at pH 7.4 for 30 min ( b ), 4% PF in PBS for 30 min and labeled for CaMKII ( c ), a cytosolic protein in neurons , and 2% PF in PBS for 15 min and labeled for IRSp53 ( d ), an actin-associated protein enriched at the PSD . All fixations were carried out at room temperature. Membranes were poorly preserved in ( d ) due to the lower concentration of PF and the shorter fixation time. Scale bars = 100 nm
Techniques Used: Comparison, Preserving, Labeling, Concentration Assay
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other:Article Title: Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains Article Snippet: Homer 2 , PSD , Article Title: Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains Article Snippet: Homer 1 , PSD , |